BioEngineering @ UQ

2004

Kelm JM, Ehler E, Schlatter S, Nielsen LK, Perriard J, Fussenegger M (2004) Design of Artificial Myocardial MicroTissues. Tissue Engineering 10: 201-14. 

2003

Fong Chong B, Nielsen LK (2003) Aerobic cultivation of Streptococcus zooepidemicus and the role of NADH oxidase. Biochemical Engineering Journal 16: 153-162. pdf

Croll T, Munro PD, Winzor DJ, Trau M, Nielsen LK (2003) Analysis of the phase behavior of the aqueous poly(ethylene glycol) - Ficoll system. Biotech Progress 19: 1269-1273. pdf

Kelm JM, Timmins NE, Brown CJ, Fussenegger M, Nielsen LK (2003) A method for the generation of homogeneous multicellular tumor spheroids applicable to a wide variety of cell types. Biotechnology and Bioengineering 83:173-180. pdf

Underhill MF, Coley C, Birch JR, Findlay A, Kallmeier R, Proud CG, James DC (2003) Engineering mRNA translation initiation to enhance transient gene expression in chinese hamster ovary cells. Biotechnology Progress 19 (1): 121-129.

Lua LHL, Reid S (2003), Effect of time of harvest of budded virus on the selection of baculovirus FP mutants in cell culture. Biotechnol Prog 19:238-242. pdf

Croll T, Munro PD, Winzor DJ, Trau M, Nielsen LK (2003) Quantitative Prediction of Phase Diagrams for Polymer Partitioning in Aqueous Two-Phase Systems. J Polymer Sci B 41:437-444. pdf

Lua LHL, Nielsen LK, Reid S (2003) SDS Sensitivity of Helicoverpa armigera Nucleopolyhedrovirus Polyhedron Envelope and Sequence of Polyhedron Envelope Protein Gene. Biological Control 26: 57-67. pdf

Fong Chong B, Nielsen LK (2003) Amplifying the cellular reduction potential of Streptococcus zooepidemicus. J. Biotechnology 100:33-41. pdf

2002

Baker KN, Rendall MH, Patel A, Boyd P, Hoare M, Freedman RB, James DC (2002) Rapid monitoring of recombinant protein products: a comparison of different technologies. Trends in Biotechnology 20(4): 149-156.

Smales CM, Pepper DS, James DC. (2002) Protein modification during anti-viral heat treatment bioprocessing of Factor VIII, Factor IX concentrates and model proteins in the presence of sucrose. Biotechnology and Bioengineering 77 (1): 37-48.

Chan LCL, Young PR, Bletchly C, Reid S (2002) Production of the baculovirus-expressed dengue virus glycoprotein NS1 can be improved dramatically with optimised regimes for fed-batch cultures and the addition of the insect moulting hormone, 20-Hydroxyecdysone. J Virol Methods 105:87-98.

Lua LHL, Pedrini MRS, Reid S, Robertson A, Tribe DE (2002) Phenotypic and genotypic analysis of Helicoverpa armigera nucleopolyhedrovirus serially passaged in cell culture. J Gen Virol 83:945-955. pdf

Rosinski M, Reid S, Nielsen LK (2002) Kinetics of baculovirus replication and release using real time quantitative PCR. Biotechnol Bioeng 77:476-80. pdf

2001

Blank LM, Koebmann BJ, Michelsen O, Nielsen LK, Jensen PR (2001) Hemin reconstitutes proton extrusion in an H+-ATPase-negative mutant of Lactococcus lactis. J Bac 183:6707-6709. pdf

Hills AE, Patel A, Boyd P, James DC. (2001) Metabolic control of recombinant monoclonal antibody N-glycosylation in GS-NS0 cells. Biotechnology and Bioengineering 75: 239-251

Smales CM, Pepper DS, James DC. (2001) Protein modifications during anti-viral heat bioprocessing and subsequent storage. Biotechnology Progress. 17: 974-978.

Davis PJ, Smales CM, James DC (2001) How can thermal processing modify the antigenicity of proteins? Allergy 56, 56-60.

Baker KN, Rendall MH, Hoare M, Freedman RB, James DC. (2001) Metabolic control of recombinant protein N-glycosylation in Chinese hamster ovary and NS0 murine myeloma cells. Biotechnology and Bioengineering 73: 188-202.

Smales CM, Pepper DS, James DC. (2001) Evaluation of protein modifications during anti-viral heat bioprocessing by electrospray ionization mass spectrometry.  Rapid Communications in Mass Spectrometry 15: 351-356.

2000

Jang JD, Sanderson CS, Chan LCL, Barford JP, Reid S (2000) Structured modeling of recombinant protein production in batch and fed-batch culture of baculovirus-infected insect cells. Cytotechnology 34:71-82.

Lua LHL, Reid S (2000) Virus morphogenesis of Helicoverpa armigera nucleopolyhedrovirus in Helicoverpa zea serum-free suspension culture. J Gen Virol 81:2531-2543. pdf

Smales CM, Pepper D, James DC. (2000) Mechanisms of protein modification during model anti-viral heat-treatment bioprocessing of beta-lactoglobulin variant A in the presence of sucrose.  Biotechnology and Applied Biochemistry 32: 109-119.

Hooker AD, James DC (2000) Analysis of glycoprotein heterogeneity by capillary electrophoresis and mass spectrometry. Molecular Biotechnology 14 (3): 241 - 249.

Smales CM, Pepper D, James DC. (2000) Protein glycation during anti-viral bioprocesses. Biotechnology and Bioengineering 67: 177-188.

Blank LM, Koebmann BJ, Nielsen LK, Jensen PR (2000) Hemin reconstitutes the growth of an H+-ATPase negative mutant of Lactococcus lactis. In: Animating the Cellular Map (ed. Hoffmeyr JS, Rohwer JM, Snoep JL). Proceedings of the 9th International Meeting on BioThermoKinetics, Stellenbosch, South Africa, April 3-7, 2000. Pp.: 291-297.

Rosinski M, Reid S, Nielsen LK (2000) Osmolarity effects on observed insect cell size after baculovirus infection are avoided using growth medium for sample dilution. Biotechnol. Prog. 16:782-785.

Nielsen LK (2000) Virus production from cell culture, kinetics [Invited Book chapter]. In: The Encyclopedia of Cell Technology (ed. Spier RE). New York: John Wiley & Sons, pp. 1217-1230.

1999

James DC, Baker K. Glycosylation during protein secretion (1999). Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis and Bioseparation. Flickinger MC, Drew SW eds. pp.1336-1349. Wiley, New York. ISBN 0471 138223.

Lagutin OV, Dobrovolsky VN,Vinogradova TV, Kyndiakov BN, Khodarovich YM Jenkins N, James DC, Markham N, Larionov OA (1999) Efficient human IFN-g expression in the mammary gland of transgenic mice.  Journal of Interferon and Cytokine Research. 19: 137-144.

Hooker AD, Green NH, Baines AJ, Bull AT, Jenkins N, Strange PG, James DC (1999) Constraints on the transport and glycosylation of recombinant IFN-g in Chinese hamster ovary and insect cells. Biotechnology and Bioengineering 63 (5): 559-572.

Nielsen LK (1999) Bioreactors for haematopoietic cell culture. Annual Review of Biomedical Engineering 1:129-152.

Desai RP, Nielsen LK, Papoutsakis ET (1999) Stoichiometric modeling of Clostridium acetobutylicum fermentations using nonlinear constraints. J. Biotechnol. 71:191-205.

1998

Goldman MH, James DC, Rendall M, Ison AP, Hoare M, Bull AT. (1998) Monitoring N-glycosylation of recombinant human interferon-g produced during perfused fluidized-bed and batch culture of CHO cells. Biotechnology and Bioengineering 60(5) 596-607.

Hooker AD, James DC (1998) Monitoring recombinant protein heterogeneity. In: Methods in Biotechnology, Vol 8: Animal Cell Biotechnology. Pp 277-288, N. Jenkins ed. Humana Press, Totowa.

Hooker AD, James DC (1998) The glycosylation heterogeneity of recombinant human interferon-g.  Journal of Interferon and Cytokine Research. 18: 287-295.

Chan LCL, Greenfield PF, Reid S (1998) Optimising fed-batch production of recombinant proteins using the baculovirus expression vector system. Biotechnol Bioeng 59:178-188.

Nielsen LK, Bender J, Miller WM and Papoutsakis ET (1998) Population balance model of in vivo neutrophil formation following bone marrow rescue therapy. Cytotechnology 28:157-162.

Collins PC, Nielsen LK, Patel SD, Papoutsakis ET, Miller WM (1998) Characterization of hematopoietic cell expansion, oxygen uptake and glycolysis in a controlled, stirred-tank bioreactor system. Biotechnol. Progr. 14:466-472.

Nielsen LK, Papoutsakis ET, Miller WM (1998) Modelling ex vivo hematopoiesis using chemical engineering metaphors. Chem. Eng. Sci., 53: 1913-1925.