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	Proteomics and Systems Biotechnology
	Protein Glycosylation and Analysis
	Cell Engineering and Gene Expression

Click to open the GS-NS0 Murine Myeloma Proteome Map

Proteins from whole cell lysates of GS-NS0 murine myeloma cells producing a recombinant monoclonal antibody have been separated by 2D polyacrylamide gel electrophoresis (2D PAGE). Isoelectric focusing was performed over a pI 3-11 non-linear gradient and subsequent second dimension separation over a 12% polyacrylamide gel. Resolved protein spots were excised and identified by mass spectrometry (1). The image presented here is one of the Sypro Ruby stained images for GS-NS0 cell line 2N2 used for quantitative analysis and has been converted to a web accessible format using the htmlmaker program gratiously provided by Junyu Li et al. (2).

Instructions

• Hover the cursor over marked spots to show the Name of the identified protein and its pI and MW (kDa) predicted by SwissProt.
• Click on the spot to open the SwissProt entry in a new window.

References

1. Smales CM*, Dinnis DM*, Stansfield SH*, Alete D, Sage EA, Birch JR, Racher AJ, Marshall CT, James DC. Comparative proteomic analysis of GS-NS0 murine myeloma cell lines with varying recombinant monoclonal antibody production rate. Biotechnology and Bioengineering 88(4): 474-488 [Medline]
2. Li J, Liu S, Osterman T, Zhang Y, Coppage H, Pedrick N, Witzmann FA. A software utility for creating interactive maps for 2D gel-based proteomics Analytical Biochemistry 332(1): 187-90 [Medline]